Glycan Modification

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Glycan introduction onto protein | modification

Three different enzymes are applied step by step and introduced GalNAc, Gal and Siain series.
This is showing that it is possible to prepare O-glycan on a recombinant protein which produced by E. coli.

Glycan_introduction_onto_protein

Glycan modifycation protein

1. Recombinant protein expressed by E.coli
1) Recombinant protein expressed by E.coli
2. GalNAc introduction by ppGalNAcT
2) GalNAc introduction by ppGalNAcT
3. Addition of GalT
3) Addition of GalT
4. Addition of Sia (Neu5AC) by SiaT
4) Addition of Sia (Neu5AC) by SiaT
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