Glycan structural analysis

HOME / Glycan structural analysis / Analysis of glycans on glycoproteins and cell surfaces

Analysis of glycans on glycoproteins and cell surfaces | Analysis

Analysis_of_glycans_on_glycoproteins_and_cell_surfaces
  • cleavage of glycans
  • purification and analysis with MS

MS analysis enable us to compare and estimate glycan structures from glycoproteins, cells and tissues. MSn analysis is suitable to determine the glycan structures in detail.

N-glycans and O-glycans are cleaved enzymatically and chemically, respectively. After their purification, the glycans are analyzed with MS. For MSn analysis, reducing termini of the glycans are labeled with a fluorescence.

PageTop